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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which ends up in chromosome instability, and so might add to tumorigenesis. This protein was found to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a critical role in advertising cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are explained. Two more splice variants were being noted but specific nucleotide sequence data is just not nevertheless out there.
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Blocking peptides are peptides that bind specially towards the concentrate on antibody and block antibody binding. These peptide usually includes the epitope identified from the antibody.
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This manual outlines even further feasible optimization of mobile and immunoassay parameters to ensure the best possible effects are...
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best final results are...
Antibodies bound to the blocking peptide not bind to the epitope to the concentrate on protein. By evaluating the staining within the blocked antibody versus the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs into the really conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein read more abundance with the mobile cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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